Improved AAV tropism is essential for gene therapy to reach its full potential. New capsid engineering techniques are taking on this challenge. Clinical translation of gene therapies has a history ...

In 2014, researchers combined DNA barcoding with next-generation sequencing (NGS) to develop the adeno-associated virus (AAV) barcode-seq technology for streamlining ...

Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems for HDR-Mediated Genome Editing in Vivo ...

Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems for HDR-Mediated Genome Editing in Vivo ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...

ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 assay simultaneously detects the AAV genome. Software analyzes the signals from each capsid type to calculate ...

Genome editing and chemical genetics in human cells. A, Adeno-associated virus (AAV) vectors are used to deliver ssDNA directly to the nucleus ... rather than in strict coordination with outer ...

Gator Bio is the market’s sole solutions supplier that provides assays for genome titer and integrity, capsid titer, and empty-full ratio on a single automated platform. The AAV genome titer and ...

As they report, optimizing SXC conditions using isocratic separation to separate empty and full AAV capsids from the lysate enabled 51.7 percent of the viral genome to be recovered. It reduced DNA ...

To overcome these barriers, researchers explored lentiviral vectors, which integrate into the genome and bypass AAV-specific limitations. In the study, "Lentiviral Gene Therapy with CD34 ...