In 2014, researchers combined DNA barcoding with next-generation sequencing (NGS) to develop the adeno-associated virus (AAV) barcode-seq technology for streamlining AAV screening. Using this method, ...
ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 assay simultaneously detects the AAV genome. Software analyzes the signals from each capsid type to calculate ...
Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems for HDR-Mediated Genome Editing in Vivo ...
As they report, optimizing SXC conditions using isocratic separation to separate empty and full AAV capsids from the lysate enabled 51.7 percent of the viral genome to be recovered. It reduced DNA ...
Gator Bio is the market’s sole solutions supplier that provides assays for genome titer and integrity, capsid titer, and empty-full ratio on a single automated platform. The AAV genome titer and ...
Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems for HDR-Mediated Genome Editing in Vivo ...