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一次反应即可同时检测目的蛋白(Akt)与内参GAPDH的含量。 右图:A-E分别表示细胞株ELC、U937、HSC、SPCA、W304总蛋白(上样量30 μg / 泳道),HRP标记β-actin单抗、HRP标记抗GAPDH单抗、HRP标记β-tubulin单抗稀释比例均为1:50,000,室温孵育1小时。超敏化学发光底物A 50 μl ...
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