CRISPR technology, driven by Cas9, redefines gene editing, facilitating targeted DNA modifications that advance genetic ...

Currently, CRISPR/Cas9 is a promising RNA-guided genome editing technology consisting of a Cas9 nuclease and a single-guide RNA (sgRNA). Considering the significant differences between lncRNAs and ...

Guided by RNA, the Cas9 endonuclease breaks DNA at a target ... using adeno-associated virus with transposon-based delivery of the CRISPR guide RNAs. Characterization of the genome editing ...

CRISPR-Cas9, a gene editing technology known for its user-friendliness, can knock genes in or out. Knocking out a gene involves inserting CRISPR-Cas9 into a cell using a guide RNA that targets the ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

Over the years, scientists have explored alternatives to Cas9, such as Cas12a for more precise multigene editing, a type III CRISPR for RNA editing, and a Cas13 loaded with multiple guide RNAs for ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on DNA replication. KDM1A promotes survival in DAC-treated cells via DNA ...

CRISPR/Cas9 works using a Cas9 enzyme and a guide RNA. Lau compared the guide RNA to a global positioning system (GPS) that scans the genome and leads the Cas9 to cut DNA at a specific sequence ...

The CRISPR-Cas gene scissors offer a wide range of potential applications, from the treatment of genetic diseases to antiviral therapies and diagnostics. However, to safely harness their powers, ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

A UCO team has applied the genome editing technique, which garnered a Nobel Prize in Chemistry in 2020, to unravel the functions of two genes involved in the metabolism of beans and that could not be ...