Researchers have uncovered a serious side effect of using the CRISPR-Cas gene scissors. A molecule designed to make the process more efficient destroys parts of the genome.

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Genome editing with various CRISPR-Cas molecule complexes has progressed rapidly in recent years. Hundreds of labs around the ...

CRISPR-Cas9 is effective at generating small mutations ... However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient.

The CRISPR-Cas9 molecular complex ... The cut activates two natural repair mechanisms that the cell uses to repair such damage: a fast but imprecise one that reconnects only the ends of the ...

The therapy molecule AZD7648 can promote precise DNA repair during CRISPR gene editing, but creates massive genetic changes ...

The complexity of the central nervous system (CNS) and its pathologies necessitates an intricate understanding of the cellular and molecular mechanisms at play. Single-cell biology has emerged as a ...