The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

CRISPR, or Clustered Regularly Interspaced Short ... This includes an approach that utilizes Cas9 nickase, which is a variant that induces single-stranded breaks in combination with a single ...

A CRISPR Cas9 nickase protein makes a single-strand DNA nick, and then a base-converting enzyme, physically attached to Cas9, makes a cytosine-to-thymine switch. We typically deliver base editors ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...