Moreover, cell lysis, required in both approaches, does not maintain the subcellular localization of phosphorylated proteins. Standard in situ protein detection protocols such as ...
The study reveals that H. borinquense attacks H. volcanii, leading to cell lysis and growth inhibition in H. volcanii. Crucially, this antagonistic behavior is contact-dependent, meaning the two ...
Suitable antibodies for immunoprecipitation of the desired protein complex and optimization of the cell line, culture conditions and methods for cell lysis and antibody affinity purification are ...