CRISPR 技术自问世以来，就如同生命科学领域的一把 “神奇剪刀”，能够精准地对基因进行编辑，为生命科学研究和医学治疗带来了革命性的变化。它可以像修改文档中的文字一样，对生物的基因组进行精确修改，这一特性使得科学家们能够深入探究基因的功能，开发新型的治疗方法。然而，这把 “剪刀” 在使用过程中却存在一些棘手的问题。设计高效且特异性强的向导 RNA（guide RNA，gRNA）成为了 ...

We next aimed to verify the dCas9-EGFP signals with FISH using probes targeting repetitive sequences so that a single FISH probe could access many sites. We used another two sgRNA sequences ...

为解决 CRISPRa 最佳转录激活机制不明的问题，浙江大学医学院附属第一医院等单位的研究人员开展转录凝聚体动态特性对 CRISPRa 介导基因激活影响的研究。发现其可调节转录爆发，明确凝聚体特性与激活关系。对优化 CRISPRa 技术意义重大，推荐阅读！ 研究由浙江 ...

CRISPR interference (CRISPRi) is a novel method for specific gene knockdown. It's CRISPR, without the cut. The Horizon CRISPRi system uses a deactivated Cas9 nuclease (dCas9) fused to our proprietary ...

CRISPR epigenome editing successfully reactivates silenced genes in Prader-Willi Syndrome, highlighting potential for ...

(Courtesy of Stanley Qi) CRISPRKit works in a similar way, though it employs dCas9, or “dead” Cas9, a deactivated version of the Cas9 enzyme unable to cut DNA. “Whereas CRISPR-Cas9 is ...

CRISPR is incredibly robust in terms of cutting DNA and targeting nucleic acids ... CRISPRa uses a version of Cas9 that is inactive (dCas9). It cannot cut DNA, but it progressively binds to targets ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...