Cy3 emits fluorescence with a peak wavelength at 563 nanometers (black line), while Cy5 at 670 nanometers (red line). In the experiment, Cy3 is attached to CB[7] and Cy5 to AdA.

is extracted and differentially labeled with compatible fluorophores (e.g., Cy3, Cy5). Equal amounts of labeled DNA is hybridized to the microarray, and the hybridization reaction is allowed to ...

Using a single probe [view schematic for single labeling], you can choose to develop the signal with a chromogenic method (alkaline phosphatase with BCIP/NBT, a purple precipitate) or fluorescence ...