The method was developed by Frederick Sanger in 1975, who was later awarded the Nobel Prize in chemistry in ... Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis.

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

(1) Protected deoxynucleotide triphosphate (dNTP) and tethered dNTP approaches both use terminal deoxynucleotidyl transferase (TdT), a unique and specialized DNA polymerase that does not require a ...

The new RNA sequences are complementary to their DNA template, rather than being identical ... attached to one another in a chain via chemical bonds called phosphodiester bonds.

During DNA replication, the double helix unwinds, and each strand serves as a template for synthesizing a new complementary strand. Adenine on the template strand will always pair with thymine on the ...