This guideline applies to all lab personnel working with ethidium bromide (EB) (CAS# 1239-45-8). Ethidium bromide is a powerful mutagen and is a skin, eye and respiratory irritant. It can be readily ...

1. Prepare a 40 ml 1 % agarose gel in 0.5X TBE, containing 0.5 µg/ml ethidium bromide. Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one ...

Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric product–formation in ABIL EM 90–based emulsion PCRs. Transfer 400 μl of the oil ...

Figure 1: Ethidium bromide-stained agarose gels of restriction digests of 0.5 μg λ-DNA, using 1 μl of serially diluted restriction enzymes (in supplied 1× buffer). If applicable to all enzymes ...