Following separation, the RNA is transferred from the gel onto a blotting membrane. (Although this step is what gives the technique the name "northern blotting," the term is typically used to ...

Northern blotting ... Rinse the gel with DEPC-treated H 2 0; soak for 20 min in five gel volumes of 0.01 N NaOH/3 M NaCl. 3| Place a piece of thick blotting paper on a sheet of Plexiglas or ...