How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...

the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time RT–PCR. Fast and accurate, real-time RT–PCR ...

Real-time PCR is the favored method for measuring gene ... But a new service provided by the National Cancer Institute will do the work for you. The Quantitative PCR Primer Database (http ...