Figure 3: 1% agarose gel tracing the large-scale purification of pBGS19Luxwt plasmid DNA. Anion-exchange chromatography is commonly used for final purification of plasmid DNA 13. It was found that ...
Prepare plasmid DNA using standard miniprep protocols ... UV absorbance at 260 nm or by running an aliquot on an agarose gel. We purify the DNA by electrophoresis through Whatman glass fiber ...