while repair pathways can be engineered to introduce specific point mutations or insertions. Single-cell CRISPR screening is a powerful method for biological discovery. Such screens genetically ...

In about a third of patients, the gene for dystrophin has small deletions or single base mutations that render it nonfunctional, which makes this gene an excellent candidate for gene editing.

This particularly aggressive variant of the disease is often caused by a single mutation, making it a good candidate for precision medicine. The researchers used the fibroblasts to create pluripotent ...

We need to know how all these mutations function individually to understand how cancer develops and progresses. CRISPR-based technologies that inactivate or alter the expression of entire genes ...

Analysis of CRISPR/Cas9 edited cells is done to check the efficacy of the system in introducing mutations (insertions, deletions, or substitutions) in the DNA sequences of the edited cell population.

Genome editing with CRISPR-Cas is often associated with the induction of mutations. However, a team of researchers from the Swiss University of Lausanne now shows that it can also be used to ...