Two-dimensional polyacrylamide gel electrophoresis (PAGE) is used for separation of complex protein mixtures by the independent parameters of isoelectric point and molecular weight. Isoelectric ...

1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers ... to resolve and migrate quite slowly in the native polyacrylamide gels. For analyzing larger complexes, use either ...