此外，蛋白在纯化过程中其构象可能发生改变，所获得的药物-靶标相互作用信息不能反映活细胞内的真实情况。而且很多蛋白很难重组表达纯化，进而限制了利用生物物理分子互作技术鉴定化合物和蛋白的相互作用。这时，也可以采用 细胞热迁移技术 (cell ...

This valuable study addresses potential roles of the master regulator of X chromosome inactivation, the Xist long non-coding RNA, in autosomal gene regulation. Using data from mouse cells, the authors ...

Figure 2: Precipitation by spermidine of 40 μg/ml pBGS19Luxwt or Baker's yeast RNA in 10 mM Tris buffer at pH 8.0 with and without 600 mM NaCl. To eliminate compaction agent from the DNA pellet ...

High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8 ...

Finally, place the binding column in a new 2 ml collection tube. 11. Pipette 100 µl of Elution Solution (Tris-EDTA buffer, pH 9.0) directly into the centre of the binding column and let sit at room ...

Human leukocyte antigen (HLA) genotyping is critical when preparing for stem cell transplants. The procedure is crucial to ...

(Fragments greater than 1 kb long are not transferred efficiently by capillary action.) The gel is then soaked in alkaline solution to denature the DNA, and then neutralized in Tris/NaCl solution. The ...

Proteins were then transferred to polyvinylidene difluoride membranes, blocked with 5% nonfat dry milk in 100 mmol/L Tris-HCl buffer, pH 7.5, with 150 mmol/L NaCl and 0.1% Tween 20 (Tris buffered ...

Explore a novel approach of structural analysis using Microfluidic Modulation Spectroscopy, offering insights into protein ...

This valuable report describes the changing antiviral activity of IFIT1 across mammals and in response to distinct viruses, likely as a result of past arms races. One of the main strengths of the ...

Portra / Getty Images A capital buffer is mandatory capital that financial institutions are required to hold in addition to other minimum capital requirements. Regulations targeting the creation ...