Discover how a novel acrylamide gel casting system allows researchers to quickly acquire high-quality results. Although hand-cast gels are far cheaper than precast gels, researchers must endure a time ...

1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...