CRISPR technology, driven by Cas9, redefines gene editing, facilitating targeted DNA modifications that advance genetic ...

Currently, CRISPR/Cas9 is a promising RNA-guided genome editing technology consisting of a Cas9 nuclease and a single-guide RNA (sgRNA). Considering the significant differences between lncRNAs and ...

Guided by RNA, the Cas9 endonuclease breaks DNA at a target ... using adeno-associated virus with transposon-based delivery of the CRISPR guide RNAs. Characterization of the genome editing ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

Over the years, scientists have explored alternatives to Cas9, such as Cas12a for more precise multigene editing, a type III CRISPR for RNA editing, and a Cas13 loaded with multiple guide RNAs for ...

One of the barriers to using CRISPR-Cas9 gene editing in the clinic is the possibility ... mutations throughout the genome and show in mice that a carefully designed guide RNA strand does not produce ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on DNA replication. KDM1A promotes survival in DAC-treated cells via DNA ...

The CRISPR-Cas gene scissors offer a wide range of potential applications, from the treatment of genetic diseases to antiviral therapies and diagnostics. However, to safely harness their powers, ...

CRISPR/Cas9 works using a Cas9 enzyme and a guide RNA. Lau compared the guide RNA to a global positioning system (GPS) that scans the genome and leads the Cas9 to cut DNA at a specific sequence ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

CRISPR/Cas9 Modifies Euglena to Create Potential Biofuel ... The team discovered that an RNA molecule, rather than a protein as ... Immune System That Bacteria Use to Respond to Viral Infections ...