The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

CRISPR, or Clustered Regularly Interspaced Short ... This includes an approach that utilizes Cas9 nickase, which is a variant that induces single-stranded breaks in combination with a single ...