GFP - LC3/RFP - SKL 转染：使用 Lipofectamine 3000 将编码 GFP - LC3 和 RFP - SKL 的质粒转染到 PTECs 中，在特定条件下培养 24h 后获取荧光图像。 组织学和免疫组化：将肾脏固定、脱水、包埋在石蜡中，切片后进行 Masson 三色染色和免疫组化，用特定抗体孵育，经显色后获取 ...

Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea ...

尽管由外向内信号传导不影响 GFP/RFP 比率，但暴露于饥饿条件下会显著降低 GFP/RFP 比率，这表明整合素依赖的 LC3 聚集不会诱导自噬通量（补充图 8d，e）。总之，这些结果表明 LC3 介导的淋巴细胞黏附与经典自噬通量的发生无关。

GFP-LC3-RFP-LC3ΔG-knockin mice were generated in collaboration with ViewSolid Biotech (Beijing, China), via the PiggyBac transposon system for efficient gene transfer. This system enables the “cut-and ...

The relationship between such endocytic/autophagic activities in early-stage embryos and the embryonic developmental potential has been recently studied. Using GFP-LC3 as an indicator of autophagic ...

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065 ,P. R. China ...

Plants mobilize the endomembrane system for restoring homeostasis when stimulated by stresses. Though the function of ufmylation in ER stress and autophagy has been described in mammals, their ...

One of the key characteristics of Bluetooth LE Audio is the new high performing standards-based LC3 audio codec. LE Audio requires the LC3 codec which supports scaling from 345kbps to just 16kbps ...

The LC3 codec algorithm for DesignWare® ARC® processors was designed as a low complexity communications codec within the Bluetooth LE Audio specification, to deliver voice and audio streaming. The LC3 ...