Sequences were pipe-cloned (30) into pcDNA5, preceded by a mouse kappa light chain leader sequence, and followed by bases coding for six histidine residues to facilitate secretion of the Fc proteins ...

Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, United States; Department of Biophysics, University of Texas Southwestern Medical Center, ...

The chimeric heavy and light chains were expressed by co-transfected HEK293F suspension cells. The IgG molecules were purified from cell culture supernatant by using Protein A resin. The purified IgG ...

Get article recommendations from ACS based on references in your Mendeley library. Pair your accounts.

药物转运体是以药物为基质，存在于组织或器官的细胞膜表面，担当药物跨膜转运功能的蛋白质的总称。药物在体内的过程包括吸收、分布、代谢、排泄，多种药物及外源性分子通过细胞膜主要是由药物转运体来控制。肾脏、肝脏和肠道是药物代谢的主要器官 ...