Avoid fusions to the GFP fragments at the site of protein-protein interaction ... roughly stoichiometric levels of expression. We find that 0–20 μM IPTG and 0.02–0.2% arabinose is useful.

Heat shock protein (HSP) 47 is a collagen-specific molecular chaperone involved in fibrotic diseases. The molecular mechanisms of HSP47 induction in intestinal fibrosis related to CD, however, remain ...

This important study identifies the "H-state" as a potential conformational marker distinguishing amyloidogenic from non-amyloidogenic light chains, addressing a critical problem in protein misfolding ...

The GST protein was purified using IPTG (isopropyl-beta-D ... staining illustrated differential expression of VSMC contractile markers (including SM22α [transgelin2] and SMMHC) and synthetic markers ...

Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey 08544, United States Omenn-Darling Bioengineering Institute, Princeton University, Princeton, New Jersey ...

In addition to reducing your overall calorie intake, increasing the amount of protein you consume may help you lose weight. Protein powders are a convenient way to add more protein to your diet ...

So you're trying to bulk up. How can boosting your protein intake boost your gym gains? The Recommended Dietary Allowance (RDA) for protein is currently 0.36 grams of protein per pound ...

Department of Molecular Biology, Kyushu University Graduate School of Pharmaceutical Sciences, Fukuoka, Japan ...

If you want to learn more about protein or change your protein goals, you should first consult your doctor. To help put things in perspective, we created this visual guide to show what 100 grams ...