The PoSM (green) was identified by PSD-GFP cryoCLEM and the PreSM (cyan) was identified by its tethering to the PoSM and the prevalence of synaptic vesicles. Salient organelle and macromolecular ...

Plasticity-related gene (PRG) 5 is a vertebrate specific membrane protein, that belongs to the family of lipid ... Subsequently, eGFP or FLAG fusion proteins (Table 1) were purified using either µMACS ...

The intracellular bacterial pathogen Coxiella burnetii secretes an effector protein, CbEPF1, which manipulates LD metabolism by forming inter-organelle contact sites between the host endoplasmic ...

The study highlighted the importance of membrane mechanics to the supercomplex formation and revealed differences in structural and dynamical features of the protein components upon complex formation.

Viruses are masters of stealth. From the moment a virus enters the host's body, it begins hijacking its cells. First, the ...

HC201) and anti-GFP mouse monoclonal antibody (Cat ... and proteins were further electrophoretically transferred to nitrocellulose membranes using the Bio-Rad TransBlot protein transfer system. The ...

2. GFP receiver inducing mCherry (imC cell): Cells that express GFP-anchor protein and anti-GFP synthetic Notch (synNotch) receptor, which recognizes GFP and induce the mCherry reporter (Fig. EV1A).

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