1）将预测能够与miRNA结合的靶基因序列（5’UTR、3’UTR、ORF区域）或者lncRNA序列（野生型或者突变体）插入报告基因载体pGreenⅡ0800-Luc上。 注：pGreenⅡ0800-Luc载体含Firefly luciferase（萤火虫萤光素酶）和Renilla luciferase（海肾萤光素酶），Renilla luciferase作为对照。

为解决牙髓炎症后骨组织修复难题，东京医科齿科大学研究人员开展 miRNA - 27a 对 hDPSCs 分化及体内成骨影响的研究。结果显示其能促进分化与成骨。该研究为骨再生提供新思路，极具科研价值，推荐一读。

Notably, this miRNA alleviates the suppressive effect of EGF on IFN-I signaling ... Subsequently, the cells were lysed with passive lysis buffer, and firefly and renilla luciferase activities were ...

Firstly, the differentially expressed miRNAs were screened by bioinformatics methods from two GEO miRNA expression profiles (GSE158195 and GSE90452 ... To confirm the TXNIP 3’-UTR as a target of ...

An amount of 150 ng luciferase reporter plasmid was cotransfected with 10 nmol/L miRNA in HEK293T cells using Lipofectamine 2000 (Thermo Fisher Scientific). Activity of Renilla and firefly luciferase ...

This study presents an important finding on the molecular mechanism for transduction of environmentally induced polyphenism. The evidence supporting the claims of the author is solid. This paper would ...

Encouragingly, the concept of miRNA-based therapeutics is developing, with synthetic antagonists of miRNAs (antagomiRs) currently in phase II trials for the treatment of chronic hepatitis C virus ...