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双荧光素酶报告基因实验原理
将海肾荧光素酶基因(hRluc)构建到另一个载体中,通常与一个恒定表达的启动子(如SV40)连接,用作内参报告基因。 细胞转染: 将上述两个载体共同转染入细胞中。实验报告基因的表达水平将受到研究的启动子或调控元件的影响,而内参报告基因的表达水平 ...
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