This valuable study proposes a novel rapid-entry mechanism of S. aureus that involves the rapid release of calcium from lysosomes. The strength of the paper lies in a very interesting hypothesis; what ...

Ramani Ramchandran, Professor at the Medical College of Wisconsin, investigates ciliary biomarkers for diagnosing and ...

CDC42 drives ciliary actin polymerization and ectocytosis. The hyperactivation of ciliary CDC42, along with excessive ...

6| Save the supernatants at 4 °C in clean microcentrifuge tubes. These samples will be analyzed by SDS-PAGE in step 9. 7| Wash the beads four times with 1 ml of ice-cold GST lysis buffer.

Low-grade inflammation was evaluated by counting cells, including intraepithelial lymphocytes (IELs), eosinophils and mast cells, and by determining the mRNA and protein expression of tumour necrosis ...

Here, we used deep full-length single-cell RNA-sequencing to distinguish post-mitotic cone and rod developmental states and cone-specific features that contribute to retinoblastomagenesis. The ...

Introduction IL-5 exerts effects on eosinophils from prolonging their survival to activation via IL-5Rα. It has been reported that binding of IL-5 by the solubilized form of the receptor, sIL-5Rα, can ...

BAL fluid cell differential counts were performed on duplicate cytospin preparations by counting 1000 cells. The supernatants were obtained by filtering the pooled BAL fluid through a 200 μm nylon ...

Correspondence to Dr Toshio Kitamura, Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo ...