This valuable study reports on the critical role of ANKRD5 (ANKEF1) in sperm motility and male fertility. However, the supporting data remain incomplete. This work will be of interest to biomedical ...

电泳分离：SDS（十二烷基硫酸钠）使蛋白质带负电荷，消除形状差异，仅按分子量在聚丙烯酰胺凝胶中分层。 转膜：通过电场将凝胶中的蛋白“印迹”到膜（PVDF或NC膜）表面，形成稳定的固相抗原。 免疫检测：一抗结合目标蛋白，二抗（偶联酶或荧光基团 ...

The sample was diluted with 5 X SDS-PAGE loading buffer and boiled for 10 min ... DEF cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X-100 for 10 min. The ...

Tissues were incubated overnight at 55°C with lysis buffer containing 100 mM Tris HCl (pH 7.8), 5 mM EDTA, 0.2% SDS, 200 mM NaCl, and 100 μg/mL proteinase ... tissue sections were immersed in PBS for ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

Soluble CD95L was then washed 3 times with lysis buffer (20 mM Tris-HCl as pH 7.6, 150 mM NaCl, 0.5% Triton X-100 and 10 μM PMSF ... an 8–16% gradient gel for SDS-PAGE and transferred to ...

（八）NLRP3 泛素化检测 慢性加暴饮乙醇喂养 10 天后，分离野生型和 小鼠的 KCs，在含有 50μM N - 乙基马来酰亚胺（Sigma-Aldrich，E3876）和蛋白酶抑制剂（Roche，04693116001）的裂解缓冲液（0.1% SDS，150mM NaCl，100mM pH 8.0 Tris-HCl，2mM EDTA 和 1% Triton X-100）中进行匀浆。裂解 ...

Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disorder caused by expansion of a polyglutamine tract in ataxin-1. In affected neurons of SCA1 patients and ...

Vivo X100 mobile was launched on 13th November 2023. The phone comes with a 120 Hz refresh rate 6.78-inch touchscreen display offering a resolution of 1260x2800 pixels and an aspect ratio of 20:9.

Fujifilm makes some of the best mirrorless cameras on the market. Last year, the company announced the Fujifilm X100VI, a mirrorless camera that joins the company's popular X100 series. Priced at ...