To alleviate the limitations discussed above, we developed a novel PCR-based application – coamplification at lower denaturation temperature (COLD)-PCR, [24] that preferentially enriches ...

Among the viruses Cucumber mosaic virus (CMV) has been rated worldwide as one of the five most important viruses infecting vegetable species. CMV is a tripartite virus with high sequence variability, ...

We have recently developed coamplification at lower denaturation temperature-PCR (COLD-PCR) to resolve this limitation. This novel form of PCR selectively amplifies low-abundance DNA variants from ...