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减数分裂是存在于有性生殖生物中的特殊细胞分裂方式，为有性生殖的基础。同源重组是减数分裂中最重要的生物学事件，它不仅使来自亲本双方的遗传物质发生交换，增加了物种遗传的多样性；而且还能让同源染色体之间建立物理连接，以确保它们的精确分离，维持染色体数量的恒 ...

Supercomputer simulations have revealed how changes in the shape of the HIV-1 capsid protein may help the virus squeeze its ...

为了解决体外重构减数分裂DSB形成这一难题，童明汉课题组利用体外蛋白表达纯化系统成功获得了SPO11-TOP6BL复合体，并首次在体外重现其切割DNA双链的活性。之后，与新华医院黄旲团队合作，采用凝胶过滤层析、交联质谱、Pull ...

▎药明康德内容团队编辑 今日，顶尖学术期刊《自然》同时上线3篇研究论文，宣告分子生物学领域内一个被誉为“圣杯”的难题终于破解。来自中国科学院分子细胞科学卓越创新中心童明汉研究员与上海交通大学医学院附属新华医院黄旲教授的合作团队，美国纪念斯隆-凯特琳癌症中心（Memorial Sloan Kettering Cancer ...

研究人员从杆状病毒感染的昆虫细胞中纯化出带有麦芽糖结合蛋白（MBP）标签的小鼠 SPO11。在体外实验中，他们将蛋白质与质粒 DNA 和二价金属离子一起孵育，结果发现 SPO11 能够产生单链（缺口）、双链（线性）和多个 DSB，这表明小鼠 SPO11 即使在没有体内所需的任何伙伴蛋白的情况下，也具有内在的双链 DNA 切割活性。当他们把 SPO11 中串联的活性位点酪氨酸残基（Y137 和 ...

Francisco Mendez Diaz is in the Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093, USA. Kevin D. Corbett is in the Department of ...

BACKGROUND: Circular RNAs derived from both nuclear and mitochondrial genomes are identified in animal cells.

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