Expression was performed in E. coli BL21 Star (DE3) on LB plates with 50 µg/mL kanamycin. Recombinant proteins were purified using Strep-Tactin affinity chromatography, and concentrations determined ...

A second concern of opponents of Amflora potatoes relates to the possibility of transgene dissemination to other potatoes. Such spreading of the transgene is unlikely, as potato transgene movement ...

For decades, poor treatment options and low-quality evidence plagued care for patients with rifampin-resistant tuberculosis. The advent of new drugs to treat tuberculosis and enhanced funding now ...

1 University of Groningen and University Medical Center Groningen, Department of Gastroenterology and Hepatology, Groningen, The Netherlands 2 University of Groningen and University Medical Center ...

Mechanical rupture, or lysis, of the cytoplasmic membrane is a common cell death pathway in bacteria occurring in response to β-lactam antibiotics. A better understanding of the cellular design ...

Mycobacterial cultures (5 mL, OD 600 = 1.0) were pelleted and resuspended in 800 μL Trizol. Cells were disrupted by bead beating. The supernatant was then extracted with chloroform:isoamyl alcohol (24 ...