Dual-luciferase reporter assay was used to test the target relationship between miR-101-3p and circ-MEMO1 or KRAS. The partial sequence in circ-MEMO1 or the 3′ untranslated region (3′ UTR) of KRAS ...

Reporter plasmid of SLC7A11 promoter double luciferase was synthesized from Generalbiol (Anhui, China) and inserted into the pGL3-Basic vector. pGL3-Basic SLC7A11 promoter was co-transfected into ...

1). AV.Luc contains only the luciferase transgene and an SV40 poly A signal. AV.SV(P)Luc is similar to AV.Luc, except that an SV40 promoter was inserted. AV.SV(P/E)Luc and AV.SV(P/SupEnh ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Internal translation initiation sites, as ...

2 National Institute for Health Research Biomedical Research Centre, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK ...

Ruptured micronuclei do not activate STING signalling. Although several transcriptional reporters of cascades downstream of STING have been used in the past, including the INFb-Luciferase reporter, ...

Cells were transfected with appropriate plasmids in 24-well plates. Cells were harvested and lysed for luciferase assays 48 hours after transfection. Luciferase assays were performed using a ...

The transcription factor of neuraminidase 3 (NEU3) of DEGs was verified by double luciferase reporting experiment, DNA pulldown, electrophoretic mobility shift assay and chromatin ...

HEK293T cells were co-transfected with a reporter construct containing the responsive element for NF-κB (pGL3-2κB luc) and pcDNA or pcDNA-FXR or pcDNA-FXRW469A ... Cells were lysed and Firefly and ...