A vast search of natural diversity has led scientists at MIT's McGovern Institute and the Broad Institute of MIT and Harvard ...

A vast search of natural diversity has led scientists at MIT's McGovern Institute and the Broad Institute of MIT and Harvard to uncover ancient ...

CRISPR technology, driven by Cas9, redefines gene editing, facilitating targeted DNA modifications that advance genetic ...

Guided by RNA, the Cas9 endonuclease breaks DNA at a target ... using adeno-associated virus with transposon-based delivery of the CRISPR guide RNAs. Characterization of the genome editing ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

Over the years, scientists have explored alternatives to Cas9, such as Cas12a for more precise multigene editing, a type III CRISPR for RNA editing, and a Cas13 loaded with multiple guide RNAs for ...

One of the barriers to using CRISPR-Cas9 gene editing in the clinic is the possibility ... mutations throughout the genome and show in mice that a carefully designed guide RNA strand does not produce ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on DNA replication. KDM1A promotes survival in DAC-treated cells via DNA ...

The CRISPR-Cas gene scissors offer a wide range of potential applications, from the treatment of genetic diseases to antiviral therapies and diagnostics. However, to safely harness their powers, ...

CRISPR/Cas9 works using a Cas9 enzyme and a guide RNA. Lau compared the guide RNA to a global positioning system (GPS) that scans the genome and leads the Cas9 to cut DNA at a specific sequence ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...