CRISPR technology, driven by Cas9, redefines gene editing, facilitating targeted DNA modifications that advance genetic ...

Call for Papers! Cas12-mediated Genome Editing in Plants . Ca. 01. Time Schedule . Submission deadline: Sunday, 30th November ...

RNA-based therapeutics have rapidly emerged over the past decade, offering a new class of medicines that differ significantly from conventional drugs. These therapies can be programmed to target or ...

撰文丨王聪编辑 | 王多鱼排版 | 水成文CRISPR-Cas 系统是细菌和古菌的适应性免疫系统，能够抵御病毒和可移动遗传元件的入侵。尽管 CRISPR-Cas9 和 CRISPR-Cas12 系统的 DNA 靶向机制已经被广泛研究，但 RNA ...

图1：CRISPR/Cas系统关键里程碑的时间轴图。 核酸分析是一项关键技术，可用于准确检测各种微生物。传统的核酸检测通常需要 ...

该方法利用肝素钠干扰 Cas12a 与 crRNA（CRISPR RNA）的结合过程，精准调节 Cas12 的顺式切割能力，等温扩增产物的积累会产生明显荧光信号。此外，这种通用检测方法适用于经典和非最优的前间区序列邻近基序（PAM），以及多种 Cas12a 亚型，如 LbCas12a、AsCas12a 和 ...

This review aims to explore the latest advancements in multiplex CRISPR genome editing for trees, assess the scientific and technical challenges, highlight case studies from other species, and examine ...

CRISPR systems such as CRISPR-Cas9 revolutionized ... Neoclease’s AI-generated Cas12, overlaid with a known Cas12, shows only 40.7 percent sequence similarity, enabling new intellectual property ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...