CRISPR epigenome editing successfully reactivates silenced genes in Prader-Willi Syndrome, highlighting potential for ...

为解决 CRISPRa 最佳转录激活机制不明的问题，浙江大学医学院附属第一医院等单位的研究人员开展转录凝聚体动态特性对 CRISPRa 介导基因激活影响的研究。发现其可调节转录爆发，明确凝聚体特性与激活关系。对优化 CRISPRa 技术意义重大，推荐阅读！ 研究由浙江 ...

为解决 CRISPRa 最佳转录激活机制不明的问题，浙江大学医学院附属第一医院等单位的研究人员开展转录凝聚体动态特性对 CRISPRa 介导基因激活影响的研究。发现其可调节转录爆发，明确凝聚体特性与激活关系。对优化 CRISPRa 技术意义重大，推荐阅读！ 研究由浙江 ...

Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, ...

We identify enhancer–promoter interactions via the Capture Hi-C method and functionally validate a subset through the CRISPR activation technique. Here, we first applied the NET-CAGE approach to ...

Regions with the highest burden of poly-GR aggregates also had high levels of tau pathology. To find the poly-GR-encoding repeat expansions within the genome, the scientists devised a ...

(Courtesy of Stanley Qi) CRISPRKit works in a similar way, though it employs dCas9, or “dead” Cas9, a deactivated version of the Cas9 enzyme unable to cut DNA. “Whereas CRISPR-Cas9 is ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...

Division of Micro and Nanosystems (MST), School of Electrical Engineering and Computer Science (EECS), KTH Royal Institute of Technology, Stockholm SE-10044, Sweden Division of Micro and Nanosystems ...

Dec. 4, 2024 — Researchers have uncovered a serious side effect of using the CRISPR-Cas gene scissors. A molecule designed to make the process more efficient destroys parts of the ...