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明明冻存的时候细胞长得好好的,怎么一复苏就死翘翘了啊???
二.冻存液的配置: 常用配置比例:培养基:血清:DMSO=7:2:1 适用于大多数细胞系,能够保证细胞在冻存过程中有较高的存活率。 血清:DMSO=9:1 适用范围:适用于需要长时间保存或者具有特别珍贵性的细胞系。血清含量高,可以更好地保护细胞,并确保其在冻存后 ...
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