For tissue IF staining, tissues were collected and fixed in 4% paraformaldehyde (PFA), the ... sperm morphology from a fertile control and abnormal spermatozoa from P10 with absent, short, coiled, ...

As previously described (44), H&E staining or Papanicolaou staining kit (Solabio ... normal sperm morphology from a fertile control and abnormal spermatozoa from P10 with absent, short, coiled, bent, ...

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Primary antibodies used for immunofluorescence staining included acetylated a-tubulin (T7451, Sigma, 1:250), ZO-1 (339100, Invitrogen, 1:100), YAP/TAZ (8418, Cell Signaling Technology, 1:150). The ...

The discovery and characterization of such components was hampered by the fact that many are lost in the process of isolation of flagella for analysis by negative staining. It was not until recently ...