GFP-LC3-RFP-LC3ΔG-knockin mice were generated in collaboration with ViewSolid Biotech (Beijing, China), via the PiggyBac transposon system for efficient gene transfer. This system enables the “cut-and ...

Autophagy was quantified by western blotting of LC3, transmission electron microscopy analyses and confocal microscopy scanning of mRFP-GFP-LC3 reporter construct. Luciferase reporter assays were ...

As the authors see no co-localization of GFP/mScarlet tagged versions of SYTL5 or RAB27A with LC3 or p62, they propose that lysosomal turnover is independent of the conventional autophagy machinery.

B6D2-Tg RBGS002Osb mice were crossed with MAP1LC3B-GFP mice to obtain Mito-RFP, EGFP-LC3 ESCs. Atg3 heterozygous mice were used to isolate Atg3 wild-type and knockout ESCs. The ESCs were isolated at ...

尽管由外向内信号传导不影响 GFP/RFP 比率，但暴露于饥饿条件下会显著降低 GFP/RFP 比率，这表明整合素依赖的 LC3 聚集不会诱导自噬通量（补充图 8d，e）。总之，这些结果表明 LC3 介导的淋巴细胞黏附与经典自噬通量的发生无关。

以及 LC3 - GFP 与线粒体标记物 Mito - RFP 的共定位增加。这一系列结果表明，线粒体去极化与 Siah2 介导的线粒体自噬激活之间存在关联，是线粒体质量控制激活的重要机制。 （二）氧糖剥夺激活 Siah2 介导的线粒体自噬，并在复氧过程中刺激皮质神经元的线粒体 ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. X-linked myopathy with excessive autophagy ...