CRISPR technology, driven by Cas9, redefines gene editing, facilitating targeted DNA modifications that advance genetic ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...

The gRNAs designed using this strategy can target both the integrated HBV DNA and cccDNA. Nevertheless, the accessibility of Cas9 protein to cccDNA is another factor for consideration in gRNA design ...

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The 1-kb left and right regions from the gRNA target site of Pxyellow gene were selected as homologous arms. These two homologous arms were PCR amplified from the cloned Pxyellow gene with a pair of ...