Sequences were pipe-cloned (30) into pcDNA5, preceded by a mouse kappa light chain leader sequence, and followed by bases coding for six histidine residues to facilitate secretion of the Fc proteins ...
Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, United States; Department of Biophysics, University of Texas Southwestern Medical Center, ...
The chimeric heavy and light chains were expressed by co-transfected HEK293F suspension cells. The IgG molecules were purified from cell culture supernatant by using Protein A resin. The purified IgG ...