The plating density of MEFs required will vary from human embryonic stem cell line to cell line. We use between 2 × 10 5 and 4 × 10 5 cells per p60 plate, depending on the cell line under study.

Could all this mean that 'true' (LIF/BMP-dependent) human ES cells have been missed simply because they are much harder to derive than nodal/FGF-dependent epiblast lines? Conditions now routinely ...

including the criteria for determining which stem cell lines it is acceptable to use. Future deliberations of the committee will address items for which additional information gathering and more ...

Bush's decision in August 2001 to permit federal financing for research on human embryonic stem (ES) cells using established ES cell lines. Stem-cell research has enormous potential value in both ...

Researchers who work with materials such as fetal tissue and human embryonic stem cells are facing new restrictions, the latest in a long line of regulations, that could impede important advances.

so human EC cells resemble human ES cells and inner cell mass cells of early human blastocysts (4). Over many years we have used EC cell lines as surrogates for the study of plurotent human ES cells ...

Human embryonic stem cells accrue changes in their genomes that could make them unusable therapeutically when cultured at length, an international team of scientists report in the October Nature ...

Our laboratory aims at exploiting recent advances in stem cell biology to develop radically new therapies for degenerative disease and cancer. The main focus in the lab is on the biology and ...

It is known and used worldwide by leading research groups and many of the world’s largest pharmaceutical companies. This conditionally immortalised human podocyte cell line was originally developed by ...

The generation of the cell line was reported today in the online edition of the journal Reproductive Biology. Dr Minger said: "We are very excited about this development. Human embryonic stem ...

This team used a different approach, adding recombinase sites to transposons – mobile genetic elements – that randomly integrated in the genomes of human cell lines and mouse embryonic stem cells.