The laboratory work was developed in the Plant Physiology Laboratory of the Department of Biological Sciences (3°59’14.2 “S 79°11’52.5 “W) of the Universidad Técnica Particular de Loja, Loja, Ecuador.

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Tips were rooted in tissue culture medium (1× Murashige and Skoog basal salt mixture, 0.5mg/L indole-3-butyric acid) to produce rooted cuttings. Initial rooted cuttings were serially cultured in the ...