The complete mitochondrial genome was amplified by using universal primers in a total volume of 20 μl using 10 μl of 2 × Taq PCR Master Mix (with Taq DNA polymerase, dNTP mixture, MgCl 2, and PCR ...

The high accuracy or fidelity of semi-conservative DNA replication is the product of nucleotide template interaction, polymerase-enhanced base selectivity, exonuclease proofreading and the post ...

Long-range PCR amplification was performed using the following primer pair: Pf, 5′-ACC CGG CAG AAG ATT GTA GAG -3′; Pr, 5′-CCC AGT GTC CGT CCT ATA TTG T-3′ in a 50-μL mixture buffer containing 5 U of ...