In the 1994-1995, this problem was solved by the development of a revolutionized technique called “MHC Tetramer”, which bind strongly to antigen-specific T cells due to an increased avidity effect.

Use of murine b2-microglobulin may reduce the stability of the reagent. Typically staining of murine CD8+ T-cells with tetramer is NOT affected by the use of human b2-microglobulin. One of the factors ...

How many naive T cells that are specific for any one peptide–MHC complex exist in an individual? How does that number influence the immune response? Answers to these questions have remained ...