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骨髓瘤细胞来源的CXCL7通过JAK/STAT3通路促进肿瘤细胞增殖和溶骨病变 ...
同时,对患者进行单样本 RNA 测序,分析细胞亚群和通路激活情况。 细胞实验:选用 U266、NCI-H929 和 RPMI 8226 等骨髓瘤细胞系进行实验。通过慢病毒感染构建 CXCL7 过表达和正常对照稳定细胞系,使用小干扰 RNA(siRNA)转染敲低 CXCL7 表达。采用 CCK-8 法检测细胞增殖 ...
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