By exploring the conservation and functional diversity of RIPK3 and related RHIM-containing proteins across vertebrates, this important work sheds light on the evolutionary dynamics of these key ...

Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603 203, Tamil Nadu, India ...

HEK293T cells were seeded in 24-well plates and transfected with pCDH-GRHL3-3×Flag or pCDH-FOXO1-3×Flag and pGL3 luciferase reporter plasmids (both 600 ng/well) together with the pCMV-Renilla control ...

24-well plates were plated with HEK-293T cells. Until the cells grow reaching 70% confluence, cells were transfected with 0.75 μg of pGL3-basic/pGL3-basic-KIF20A luciferase reporter plasmid, along ...

The pGL3-Basic vector, pRL-TK luciferase reporter plasmid, and Dual-Glo Luciferase Assay System were purchased from Promega. A 1,909 base pairs (bp) of the FBXW8 promoter sequence (designated P1) and ...