This was inserted into a pUC57-Kan cloning vector following AgeI and NheI cleavage of the backbone and Geneblock and ligation by T4 DNA ligase (New England Biotechnologies) following manufacturer ...

DNA of African swine fever virus (ASFV) was a gift from other laboratory. PCV3 ORF2 gene was synthesized and cloned into the pUC57 vector by Tsingke Biological Technology (China). The synthetic ...