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生物通
21 天
CDCA7增强STAT3转录活性,调节有氧糖酵解,促进胰腺癌进展和吉西他滨 ...
(三)细胞培养 选用人胰腺细胞系 HPNE、CFPAC-1、BxPC-3、MIA PaCa-2、SW1990 和 PANC-1,分别在含 10% FBS 的 RPMI-1640 培养基或 DMEM 培养基中,于 37°C、5% CO?的培养箱中培养,通过 STR 分型鉴定细胞系并排除支原体污染。 研究人员首先通过生物信息学分析和临床样本 ...
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