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应用广场 — Inclusion body
是Sepharose 类介质的基质),它的排阻极限与Sepharose 4B 相似(对球形蛋白质为20 MDa)。柱子用含0.1% Berol 185 的破碎液来平衡。因为它的排阻极限很大,可以让其它蛋白,核酸及其它细胞器等渗入凝胶内部,而只将包涵体蛋白质排阻在外。这样只收集在空体积V0 流出的 ...
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