Adenovirus Biology, Recombinant Adenovirus, and Adenovirus Usage …
2021年12月14日 · Here, we review the biology of wild-type adenoviruses, the methodological principle for constructing recombinant adenoviruses, therapeutic applications of recombinant adenoviruses, and new technologies in pluripotent stem cell (PSC)-based regenerative medicine.
A protocol for rapid generation of recombinant adenoviruses using …
2007年5月17日 · Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We have developed an approach that simplifies the generation and production of such...
Recombinant adenovirus serotype 26 (Ad26) and Ad35 vaccine …
We demonstrate partial protection against EBOV by a single-shot Ad26 vaccine and complete protection when this vaccine is boosted by Ad35 1 month later. Increases in efficacy are paralleled by substantial increases in T- and B-cell responses to EBOV GP.
Construction, production, and purification of recombinant adenovirus ...
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. In this chapter, a standard procedure for their generation and small-scale production is described. Homologous recombination in E. coli between shuttle plasmids and full-length adenovirus ba …
A simplified system for generating recombinant adenoviruses
1998年3月3日 · Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such virus...
Recombinant human adenovirus type 5 promotes anti-tumor
2024年7月19日 · Recombinant human type 5 adenovirus (H101) is an oncolytic virus used to treat nasopharyngeal carcinoma. Owing to the deletion of the E1B-55kD and E3 regions, H101 is believed to selectively...
Production of recombinant adeno-associated virus 5 using a …
2024年9月12日 · Recombinant adeno-associated virus (rAAV) has become a popular vector for clinical use. 1 The parvovirus is capable of transduction of many different tissues and can deliver cargo for sustained expression. 2 Given the widespread use of rAAV for gene therapies, an increasing amount of emphasis has been placed on the manufacturing processes used ...
Adenovirus Vector System: Construction, History and Therapeutic ...
2022年12月7日 · AdV genome manipulation using bacterial artificial chromosome (BAC) technology has proven to be a successful alternative to conventional genetic engineering of recombinant AdVs. BAC technology enables the construction of recombinant genomes using a small amount of AdV DNA isolated from early passage isolates.
Genomic analyses of human adenoviruses unravel novel recombinant …
2021年12月15日 · Our findings confirm that recombination among HAdVs is important for molecular evolution and emergence of new strains. Therefore, further research on HAdVs, particularly among susceptible groups,...
Immunogenicity and safety of a recombinant adenovirus type-5 …
2020年8月15日 · Quantifying adenovirus-neutralizing antibodies by luciferase transgene detection: addressing preexisting immunity to vaccine and gene therapy vectors
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